Probing Universal Protein Dynamics Using Hydrogen–Deuterium Exchange Mass Spectrometry-Derived Residue-Level Gibbs Free Energy

Analyzing protein dynamics using hydrogen exchange mass spectrometry.

All cellular processes depend on the functionality of proteins. Although the functionality of a given protein is the direct consequence of its unique amino acid sequence, it is only realized by the folding of the polypeptide chain into a single defined three-dimensional arrangement or more commonly into an ensemble of interconverting conformations. Investigating the connection between protein c...

Probing protein-protein interactions with mass spectrometry.

Hydrogen exchange mass spectrometry for the analysis of protein dynamics.

Hydrogen exchange coupled to mass spectrometry (MS) has become a valuable analytical tool for the study of protein dynamics. By combining information about protein dynamics with more classical functional data, a more thorough understanding of protein function can be obtained. In many cases, protein dynamics are directly related to specific protein functions such as conformational changes during...

Probing Bunyavirus N protein oligomerisation using mass spectrometry

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Gibbs Free Energy Formula for Protein Folding

Proteins are life’s working horses and nature’s robots. They participate in every life process. They form supporting structures of cell, fibre, tissue, and organs; they are catalysts, speed up various life critical chemical reactions; they transfer signals so that we can see, hear, and smell; they protect us against intruders such as bacteria and virus; they regulate life cycles to keep that ev...